Radiolysis of Aqueous Solutions of Sugar Phosphates

Authors: N. K. Kochetkov, L. I. Kudrjashov, M. A. Chlenov, L. P. Grineva

The mechanism of the radiation-induced dephosphorylation reaction was investigated by studying the γ-radiolysis of 10mM solutions of D-glucopyranosyl phosphate, D-glucose 6-phosphate (Glc-6-P), and d-ribose 5-phosphate (Rib-5-P). Dephosphorylation occurred with OH-radical participation, since G(H3PO4) values for nitrous oxide-saturated solutions were 4.1, 1.7, and 2.3, and for nitrogen-saturated solutions 2.6, 1.1, and 1.6, respectively. The formation of phosphate-free compounds accompanied the release of inorganic phosphate. The main, neutral products of the radiolysis of Glc-6-P were 6-deoxyhexos-5-ulose (G = 0.2) and D-gluco-hexodialdose (G = 0.3). Irradiation of Rib-5-P gave ribo-pentodialdose and 5-deoxypentos-4-ulose as the main, neutral products. A scheme for the dephosphorylation process is proposed.

https://doi.org/10.1016/S0008-6215%2800%2984849-1